钢笔换墨水:【整理】免疫组化技术资料大全(原理、步骤、注意事项、教程及常见问题解答) - 丁香园论坛-...

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1 相关PPT教程
① 个人收集的免疫组化技术资料: http://www.dxy.cn/bbs/post/view?bid=68&id=9711413&sty=1&tpg=1&age=0
②免疫组化幻灯片: http://www.dxy.cn/bbs/post/view?bid=68&id=5809790&tpg=1&ppg=1&sty=1&age=0#5809790 http://www.dxy.cn/bbs/post/view?bid=49&id=10986089&tpg=1&ppg=1&sty=1&age=0#10986089 http://www.dxy.cn/bbs/post/view?bid=68&id=11008627&tpg=1&ppg=1&sty=1&age=0#11008627
③免疫组化系列幻灯: http://www.dxy.cn/bbs/actions/archive/post/5046777_1.html http://www.dxy.cn/bbs/post/view?bid=163&id=7524964&tpg=1&ppg=1&sty=1&age=0#7524964

2 基础知识
①免疫组化基础知识: http://www.bioon.com/experiment/imm6/243697.shtml
②免疫组化实验方法: http://www.bioon.com/experiment/77078.shtml
③免疫组化的试剂准备: http://www.bioon.com/experiment/imm6/290304.shtml
④Introduction to Immunocytochemistry: http://www.bioon.com/experiment/imm4/59973.shtml
⑤免疫酶细胞化学: http://www.bioon.com/experiment/imm4/8674.shtml
⑥免疫细胞化学常用试剂介绍: http://www.bioon.com/experiment/imm4/8652.shtml
⑦免疫组织化学技术介绍(酶标、荧光、胶体金): http://51protocol.com/pro/immu/immu1/20071120/43757.html
⑧免疫组化技术之免疫酶法: http://51protocol.com/pro/immu/immu1/20071112/41301.html
⑨免疫组化基本原理: http://51protocol.com/pro/immu/immu1/20071112/41296.html
⑩免疫酶组织化学技术: http://51protocol.com/pro/immu/immu1/20071102/38825.html
11)免疫组化相关理论-术语: http://51protocol.com/pro/immu/immu1/20071102/38823.html
12)免疫细胞化学常用试剂-酶消化液、封固剂、粘附剂、显色液、缓冲液、固定剂、其它: http://51protocol.com/pro/immu/immu1/20071102/38821.html http://51protocol.com/pro/immu/immu1/20071102/38820.html http://51protocol.com/pro/immu/immu1/20071102/38819.html http://51protocol.com/pro/immu/immu1/20071102/38818.html http://51protocol.com/pro/immu/20071102/38817.html http://51protocol.com/pro/immu/20071102/38816.html http://51protocol.com/pro/immu/immu1/20071102/38822.html
13)免疫组化的试剂准备: http://51protocol.com/pro/immu/immu1/20070514/29557.html
14)免疫组化技术资料集锦: http://www.dxy.cn/bbs/post/view?bid=68&id=9711413&sty=1&tpg=1&age=0

3 标准操作规程
①免疫组化步骤(ABC法): http://www.bioon.com/experiment/imm6/307114.shtml http://51protocol.com/pro/immu/immu1/20070914/37252.html
②免疫组化SP法步骤: http://www.bioon.com/experiment/imm6/307113.shtml http://51protocol.com/pro/immu/immu1/20070914/37251.html
③免疫组化(Elivison二步法): http://www.bioon.com/experiment/imm6/307111.shtml http://51protocol.com/pro/immu/immu1/20070914/37249.html
④免疫组化的经验总结(2)-操作规程(SP法/SABC法): http://www.bioon.com/experiment/imm6/275560.shtml
⑤石蜡切片免疫组化染色方法(SP法,也含冰冻切片): http://www.bioon.com/experiment/imm6/255747.shtml
http://www.bioon.com/experiment/imm6/243696.shtml
⑥免疫细胞化学染色操作规程: http://www.bioon.com/experiment/imm4/287464.shtml
⑦免疫组化双染实验protocol: http://www.bioon.com.cn/protocol/showarticle.asp?newsid=6939&typename=免疫学&sortid=1656&typeid=0

4 操作要点与技巧
①组织蜡块切片的保存: http://www.bioon.com/experiment/imm6/290306.shtml
②疫组化主要步骤: http://www.bioon.com/experiment/imm6/288778.shtml http://51protocol.com/pro/immu/immu1/20070410/9354.html
③免疫组化的经验总结(3)-操作要点和技巧: http://www.bioon.com/experiment/imm6/275561.shtml
④免疫组化实验过程中的要点和技巧: http://www.bioon.com/experiment/imm6/185836.shtml
⑤抗体的保存: http://www.bioon.com/experiment/imm4/51866.shtml
⑥免疫组化染色注意事项: http://51protocol.com/pro/immu/immu1/20071112/41305.html
⑦免疫组化技术的关键问题: http://51protocol.com/pro/immu/immu1/20071112/41304.html
⑧免疫病理学技术应注意的关键问题: http://51protocol.com/pro/immu/immu1/20071102/38828.html
⑨免疫组化经验交流: http://www.dxy.cn/bbs/post/view?bid=156&id=12002239&sty=1

5 常见问题及其解决方法
①免疫组化具体步骤中的一些问题: http://www.bioon.com/experiment/imm6/307109.shtml
②免疫组化的经验总结(1)-常见问题: http://www.bioon.com/experiment/imm6/275558.shtml
③免疫组化问题及解决办法: http://www.bioon.com/experiment/imm6/255748.shtml
http://www.bioon.com/experiment/imm6/86150.shtml
④免疫组化常见问题及解决方法: http://www.bioon.com/experiment/imm6/244363.shtml
⑤免疫组化非特异性背景染色及其控制方法: http://www.bioon.com/experiment/imm6/255746.shtml
⑥免疫组化染色过程中存在的问题、原因分析及对策: http://www.bioon.com/experiment/imm6/185844.shtml
⑦免疫组化常见问题的处理: http://www.bioon.com/experiment/imm6/86152.shtml
⑧免疫组化非特异性染色的消除方法: http://www.bioon.com/experiment/imm6/86148.shtml
⑨Immunocytochemistry: Common problems and some answers: http://www.bioon.com/experiment/imm4/59983.shtml
⑩免疫细胞化学与图像分析: http://www.bioon.com/experiment/imm4/8676.shtml
11)免疫组织化学问题解答(3): http://51protocol.com/pro/immu/immu1/20071120/43759.html
12)免疫组化成功的经验和失败的教训汇总: http://www.dxy.cn/bbs/post/view?bid=68&id=11841263&tpg=1&ppg=1&sty=1&age=0#11841263
13)免疫组化问题大全: http://www.dxy.cn/bbs/post/view?bid=68&id=9411748&sty=1&tpg=1&age=0 http://www.dxy.cn/bbs/post/view?bid=68&id=6683986&sty=1&tpg=1&age=0 http://www.bbioo.com/bio101/2005/5053.htm
14)求国外用光密度定量免疫组化的文献: http://www.dxy.cn/bbs/post/view?bid=68&id=12024797&sty=1
15)SP法和SABC法的区别: http://www.dxy.cn/bbs/post/view?bid=68&id=11997239&sty=1
16)免疫组化相关问题: http://www.dxy.cn/bbs/post/view?bid=68&id=6683986&tpg=1&ppg=1&sty=1&age=0#6683986

6 应用、进展和其它
①免疫细胞化学在神经科学中的应用: http://www.bioon.com/experiment/imm4/8660.shtml
②免疫细胞化学技术的某些新进展: http://www.bioon.com/experiment/imm4/2223.shtml
③免疫组化技术优点: http://51protocol.com/pro/immu/immu1/20071112/41297.html